NASA Logo

NTRS

NTRS - NASA Technical Reports Server

Back to Results
The C-terminal region of alpha-crystallin: involvement in protection against heat-induced denaturationRecent studies have demonstrated that the alpha-crystallins can protect other proteins against heat-induced denaturation and aggregation. To determine the possible involvement of the C-terminal region in this activity, the alpha-crystallins were subjected to limited tryptic digestion, and the amount of cleavage from the N-terminal and C-terminal regions of the alpha-A and alpha-B crystallin chains was assessed using antisera specific for these regions. Limited tryptic digestion resulted in cleavage only from the C-terminal region of alpha-A crystallin. This trypsin-treated alpha-A crystallin preparation showed a decreased ability to protect proteins from heat-induced aggregation using an in vitro assay. Together, these results demonstrate that the C-terminal region of alpha-A crystallin is important for its ability to protect against heat-induced aggregation, which is consistent with the hypothesis that post-translational changes that are known to occur at the C-terminal region may have significant effects on the ability of alpha-A crystallin to protect against protein denaturation in vivo.
Document ID
20050000424
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
Authors
Takemoto, L.
(Kansas State University Manhattan 66506)
Emmons, T.
Horwitz, J.
Spooner, B. S.
Date Acquired
August 22, 2013
Publication Date
September 1, 1993
Publication Information
Publication: The Biochemical journal
Volume: 294 ( Pt 2)
ISSN: 0264-6021
Subject Category
Life Sciences (General)
Distribution Limits
Public
Copyright
Other
Keywords
NASA Discipline Cell Biology
Non-NASA Center

Available Downloads

There are no available downloads for this record.
No Preview Available