NASA Logo

NTRS

NTRS - NASA Technical Reports Server

Back to Results
Sequence and structural implications of a bovine corneal keratan sulfate proteoglycan core protein. Protein 37B represents bovine lumican and proteins 37A and 25 are uniqueAmino acid sequence from tryptic peptides of three different bovine corneal keratan sulfate proteoglycan (KSPG) core proteins (designated 37A, 37B, and 25) showed similarities to the sequence of a chicken KSPG core protein lumican. Bovine lumican cDNA was isolated from a bovine corneal expression library by screening with chicken lumican cDNA. The bovine cDNA codes for a 342-amino acid protein, M(r) 38,712, containing amino acid sequences identified in the 37B KSPG core protein. The bovine lumican is 68% identical to chicken lumican, with an 83% identity excluding the N-terminal 40 amino acids. Location of 6 cysteine and 4 consensus N-glycosylation sites in the bovine sequence were identical to those in chicken lumican. Bovine lumican had about 50% identity to bovine fibromodulin and 20% identity to bovine decorin and biglycan. About two-thirds of the lumican protein consists of a series of 10 amino acid leucine-rich repeats that occur in regions of calculated high beta-hydrophobic moment, suggesting that the leucine-rich repeats contribute to beta-sheet formation in these proteins. Sequences obtained from 37A and 25 core proteins were absent in bovine lumican, thus predicting a unique primary structure and separate mRNA for each of the three bovine KSPG core proteins.
Document ID
20050000455
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
Authors
Funderburgh, J. L.
(Kansas State University Manhattan 66506)
Funderburgh, M. L.
Brown, S. J.
Vergnes, J. P.
Hassell, J. R.
Mann, M. M.
Conrad, G. W.
Spooner, B. S.
Date Acquired
August 22, 2013
Publication Date
June 5, 1993
Publication Information
Publication: The Journal of biological chemistry
Volume: 268
Issue: 16
ISSN: 0021-9258
Subject Category
Life Sciences (General)
Funding Number(s)
CONTRACT_GRANT: 5-P30-EY08089
CONTRACT_GRANT: EY00952
CONTRACT_GRANT: EY08104
Distribution Limits
Public
Copyright
Other
Keywords
NASA Discipline Cell Biology
Non-NASA Center

Available Downloads

There are no available downloads for this record.
No Preview Available