NASA Logo

NTRS

NTRS - NASA Technical Reports Server

Back to Results
Interstitial space and collagen alterations of the developing rat diaphragmThe effect of growth on the relative interstitial space [%total cross-sectional area (CSA)] and collagen content of the rat diaphragm muscle was examined at postnatal ages of 0, 7, 14, and 21 days as well as in adult males. The proportion of interstitial space relative to total muscle CSA was determined by computerized image analysis of lectin-stained cross sections of diaphragm muscle. To assess collagen content and extent of collagen maturation (i.e., cross-linking), high-pressure liquid chromatography analysis was used to measure hydroxyproline concentration and the nonreducible collagen cross-link hydroxylysylpyridinoline (HP), respectively. At birth, interstitial space accounted for approximately 47% of total diaphragm muscle CSA. During postnatal growth, the relative contribution of interstitial space decreased such that by adulthood the interstitial space accounted for approximately 18% of total muscle CSA. The change in relative interstitial space occurred without a concomitant change in hydroxyproline concentration. However, the concentration of HP markedly increased with age such that the adult diaphragm contained approximately 17 times more HP than at birth. These results indicate that during development the relative CSA occupied by interstitial space decreases as muscle fiber size increases. However, the reduction in relative interstitial space is not associated with a change in collagen concentration. Thus collagen density in the interstitial space may increase with age. It is possible that the observed changes in relative interstitial space and collagen influence the passive length-force properties of the diaphragm.
Document ID
20050000482
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
Authors
Gosselin, L. E.
(Mayo Clinic and Foundation Rochester, Minnesota 55905)
Martinez, D. A.
Vailas, A. C.
Sieck, G. C.
Date Acquired
August 22, 2013
Publication Date
May 1, 1993
Publication Information
Publication: Journal of applied physiology (Bethesda, Md. : 1985)
Volume: 74
Issue: 5
ISSN: 8750-7587
Subject Category
Aerospace Medicine
Funding Number(s)
CONTRACT_GRANT: HL-34817
CONTRACT_GRANT: HL-37680
Distribution Limits
Public
Copyright
Other
Keywords
NASA Program Space Physiology and Countermeasures
NASA Discipline Number 26-10
NASA Discipline Musculoskeletal
Non-NASA Center

Available Downloads

There are no available downloads for this record.
No Preview Available