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Mechanical stimulation of skeletal muscle generates lipid-related second messengers by phospholipase activationRepetitive mechanical stimulation of cultured avian skeletal muscle increases the synthesis of prostaglandins (PG) E2 and F2 alpha which regulate protein turnover rates and muscle cell growth. These stretch-induced PG increases are reduced in low extracellular calcium medium and by specific phospholipase inhibitors. Mechanical stimulation increases the breakdown rate of 3H-arachidonic acid labelled phospholipids, releasing free 3H-arachidonic acid, the rate-limiting precursor of PG synthesis. Mechanical stimulation also increases 3H-arachidonic acid labelled diacylglycerol formation and intracellular levels of inositol phosphates from myo-[2-3H]inositol labelled phospholipids. Phospholipase A2 (PLA2), phosphatidylinositol-specific phospholipase C (PLC), and phospholipase D (PLD) are all activated by stretch. The stretch-induced increases in PG production, 3H-arachidonic acid labelled phospholipid breakdown, and 3H-arachidonic acid labelled diacylglycerol formation occur independently of cellular electrical activity (tetrodotoxin insensitive) whereas the formation of inositol phosphates from myo-[2-3H]inositol labelled phospholipids is dependent on cellular electrical activity. These results indicate that mechanical stimulation increases the lipid-related second messengers arachidonic acid, diacylglycerol, and PG through activation of specific phospholipases such as PLA2 and PLD, but not by activation of phosphatidylinositol-specific PLC.
Document ID
20050000493
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
External Source(s)
Authors
Vandenburgh, H. H.
(Brown University Providence, Rhode Island 02912)
Shansky, J.
Karlisch, P.
Solerssi, R. L.
Date Acquired
August 22, 2013
Publication Date
April 1, 1993
Publication Information
Publication: Journal of cellular physiology
Volume: 155
Issue: 1
ISSN: 0021-9541
Subject Category
Life Sciences (General)
Funding Number(s)
CONTRACT_GRANT: AR39998
Distribution Limits
Public
Copyright
Other
Keywords
NASA Discipline Number 40-40
NASA Program Space Biology
Non-NASA Center
NASA Discipline Musculoskeletal

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