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Identification of a mouse B-type cyclin which exhibits developmentally regulated expression in the germ lineTo begin to examine the function of cyclins in mammalian germ cells, we have screened an adult mouse testis cDNA library for the presence of B-type cyclins. We have isolated cDNAs that encode a murine B-type cyclin, which has been designated cycB1. cycB1 was shown to be expressed in several adult tissues and in the midgestation mouse embryo. In the adult tissues, the highest levels of cycB1 transcripts were seen in the testis and ovary, which contain germ cells at various stages of differentiation. The major transcripts corresponding to cycB1 are 1.7 and 2.5 kb, with the 1.7 kb species being the predominant testicular transcript and the 2.5 kb species more abundant in the ovary. Examination of cDNAs corresponding to the 2.5 kb and 1.7 kb mRNAs revealed that these transcripts encode identical proteins, differing only in the polyadenylation signal used and therefore in the length of their 3' untranslated regions. Northern blot and in situ hybridization analyses revealed that the predominant sites of cycB1 expression in the testis and ovary were in the germinal compartment, particularly in early round spermatids in the testis and growing oocytes in the ovary. Thus cycB1 is expressed in both meiotic and postmeiotic cells. This pattern of cycB1 expression further suggests that cycB1 may have different functions in the two cell types, only one of which correlates with progression of the cell cycle.
Document ID
20050000586
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
External Source(s)
Authors
Chapman, D. L.
(Columbia University College of Physicians and Surgeons New York, New York)
Wolgemuth, D. J.
Date Acquired
August 22, 2013
Publication Date
November 1, 1992
Publication Information
Publication: Molecular reproduction and development
Volume: 33
Issue: 3
ISSN: 1040-452X
Subject Category
Life Sciences (General)
Funding Number(s)
CONTRACT_GRANT: P50 HD 05077
Distribution Limits
Public
Copyright
Other
Keywords
NASA Discipline Developmental Biology
Non-NASA Center

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