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Identification of a major continuous epitope of human alpha crystallinHuman lens proteins were digested with trypsin or V8 protease, and the resulting peptides resolved on a C18 reverse phase column. Fractions from this column were probed with polyclonal antiserum made against the whole alpha crystallin molecule. Peptides in the seropositive fraction were purified to homogeneity, then characterized by mass spectral analysis and partial Edman degradation. The tryptic and V8 digests contained only one seropositive peptide that was derived from the C-terminal region of the alpha-A molecule. To determine the exact boundaries of the epitope, various size analogues of this region were synthesized and probed with anti-alpha serum. Together, these studies demonstrate that the major continuous epitope of the alpha-A chain includes the sequence KPTSAPS, corresponding to residues 166-172 of the human alpha-A crystallin chain.
Document ID
20050000722
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
Authors
Takemoto, L.
(Kansas State University Manhattan 66506)
Emmons, T.
Spooner, B. S.
Date Acquired
August 22, 2013
Publication Date
May 1, 1992
Publication Information
Publication: Investigative ophthalmology & visual science
Volume: 33
Issue: 6
ISSN: 0146-0404
Subject Category
Life Sciences (General)
Distribution Limits
Public
Copyright
Other
Keywords
Non-NASA Center
NASA Discipline Cell Biology

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