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Proton transfer and energy coupling in the bacteriorhodopsin photocycleA description of the rate constants and the energetics of the elementary reaction steps of the photocycle of bacteriorhodopsin has been helpful in understanding the mechanism of proton transport in this light-driven pump. The evidence suggests a single unbranched reaction sequence, BR-hv----K in equilibrium with L in equilibrium with M1----M2 in equilibrium with N in equilibrium with O----BR, where coupling to the proton-motive force is at the energetically and mechanistically important M1----M2 step. The consequences of site-specific mutations expressed homologously in Halobacterium halobium have revealed characteristics of the Schiff base deprotonation in the L----M1 reaction, the reorientation of the Schiff base from the extracellular to the cytoplasmic side in the M1----M2 reaction, and the reprotonation of the Schiff base in the M2----N reaction.
Document ID
20050000740
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
External Source(s)
Authors
Lanyi, J. K.
(University of California Irvine 92717)
Date Acquired
August 22, 2013
Publication Date
April 1, 1992
Publication Information
Publication: Journal of bioenergetics and biomembranes
Volume: 24
Issue: 2
ISSN: 0145-479X
Subject Category
Life Sciences (General)
Funding Number(s)
CONTRACT_GRANT: GM 29498
Distribution Limits
Public
Copyright
Other
Keywords
NASA Discipline Exobiology
Review, Tutorial
Non-NASA Center
Review

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