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Experiments with osteoblasts cultured under hypergravity conditionsTo understand further the role of gravity in osteoblast attachment, osteoblasts were subjected to hypergravity conditions in vitro. Scanning electron microscopy of all confluent coverslips from FPA units show that the number of attached osteoblasts was similar among gravitational levels and growth durations (~90 cells/microscopic field). Specifically, confluent 1.0 G control cultures contained an average of 91 +/- 8 cells/field, 3.3 G samples had 88 +/- 8 cells/field, and 4.0 G cultures averaged 90 +/- 7 cells/field. The sparsely plated cultures assessed by immunohistochemistry also had similar numbers of cells at each time point (l.0 G was similar to 3.3 and 4.0 G), but cell number changed from one time point to the next as those cells proliferated. Immunohistochemistry of centrifuged samples showed an increase in number (up to 160% increase) and thickness (up to 49% increase) of actin fibers, a decrease in intensity of fibronectin fluorescence (18-23% decrease) and an increase in number of vinculin bulbs (202-374% increase in number of vinculin bulbs/area). While hypergravity exposure did not alter the number of attached osteoblasts, it did result in altered actin, fibronectin, and vinculin elements, changing some aspects of osteoblast- substrate adhesion.
Document ID
20050164128
Acquisition Source
Goddard Space Flight Center
Document Type
Reprint (Version printed in journal)
Authors
Kacena, Melissa A.
(Yale University School of Medicine New Haven, CT, United States)
Todd, Paul
Gerstenfeld, Louis C.
Landis, William J.
Date Acquired
August 23, 2013
Publication Date
January 1, 2004
Publication Information
Publication: Microgravity science and technology
Volume: 15
Issue: 1
ISSN: 0938-0108
Subject Category
Aerospace Medicine
Funding Number(s)
CONTRACT_GRANT: NAGW-1197
CONTRACT_GRANT: NAG5-7789
CONTRACT_GRANT: NGT5-1421
Distribution Limits
Public
Copyright
Other
Keywords
NASA Program Fundamental Space Biology
NASA Discipline Cell Biology
Non-NASA Center

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