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Escherichia coli growth under modeled reduced gravityBacteria exhibit varying responses to modeled reduced gravity that can be simulated by clino-rotation. When Escherichia coli was subjected to different rotation speeds during clino-rotation, significant differences between modeled reduced gravity and normal gravity controls were observed only at higher speeds (30-50 rpm). There was no apparent affect of removing samples on the results obtained. When E. coli was grown in minimal medium (at 40 rpm), cell size was not affected by modeled reduced gravity and there were few differences in cell numbers. However, in higher nutrient conditions (i.e., dilute nutrient broth), total cell numbers were higher and cells were smaller under reduced gravity compared to normal gravity controls. Overall, the responses to modeled reduced gravity varied with nutrient conditions; larger surface to volume ratios may help compensate for the zone of nutrient depletion around the cells under modeled reduced gravity.
Document ID
20050164137
Acquisition Source
Ames Research Center
Document Type
Reprint (Version printed in journal)
Authors
Baker, Paul W.
Meyer, Michelle L.
Leff, Laura G.
Date Acquired
August 23, 2013
Publication Date
January 1, 2004
Publication Information
Publication: Microgravity science and technology
Volume: 15
Issue: 4
ISSN: 0938-0108
Subject Category
Aerospace Medicine
Funding Number(s)
CONTRACT_GRANT: NAG2-1497
Distribution Limits
Public
Copyright
Other
Keywords
Non-NASA Center
NASA Program Fundamental Space Biology
NASA Discipline Evolutionary Biology

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