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Carnobacterium pleistocenium sp. nov., a novel psychrotolerant, facultative anaerobe isolated from permafrost of the Fox Tunnel in AlaskaA novel, psychrotolerant, facultative anaerobe, strain FTRl, was isolated from Pleistocene ice from the permafrost tunnel in Fox, Alaska. Gram-positive, motile, rod-shaped cells were observed with sizes 0(raised dot)6-0(raised dot)7 x 0(raised dot)9-1(raised dot)5 microns. Growth occurred within the pH range 6(raised dot)5-9(raised dot)5 with optimum growth at pH 7(raised dot)3-7(raised dot)5. The temperature range for growth of the novel isolate was 0-28 C and optimum growth occurred at 24 C. The novel isolate does not require NaCl; growth was observed between 0 and 5% NaCl with optimum growth at 0(raised dot)5% (w/v). The novel isolate was a catalase-negative chemoorganoheterotroph that used as substrates sugars and some products of proteolysis. The metabolic end products were acetate, ethanol and CO2. Strain FTRl was sensitive to ampicillin, tetracycline, chloramphenicol, rifampicin, kanamycin and gentamicin. 16s rRNA gene sequence analysis showed 99(raised dot)8% similarity between strain FTR1 and Carnobacterium alterfunditum, but DNA-DNA hybridization between them demonstrated 39 plus or minus 1(raised dot)5% relatedness. On the basis of genotypic and phenotypic characteristics, it is proposed that strain FTRl (= ATCC BAA-754T= JCM 12174T=CIP 108033) be assigned to the novel species Carnobacterium pleistocenium sp. nov.
Document ID
20050165084
Acquisition Source
Marshall Space Flight Center
Document Type
Reprint (Version printed in journal)
External Source(s)
Authors
Pilkuta, Elena V.
(National Space Science and Technology Center Huntsville, AL, United States)
Marsic, Damien
(Alabama Univ. Huntsville, AL, United States)
Bej, Asim
(Alabama Univ. Birmingham, AL, United States)
Tang, Jane
(American Type Culture Collection Manassas, VA, United States)
Krader, Paul
(American Type Culture Collection Manassas, VA, United States)
Hoover, Richard B.
(National Space Science and Technology Center Huntsville, AL, United States)
Date Acquired
August 23, 2013
Publication Date
January 1, 2005
Publication Information
Publication: International Journal of Systematic and Evolutionary Microbiology
Volume: 55
Subject Category
Exobiology
Distribution Limits
Public
Copyright
Other

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