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Simulation experiments of the effect of space environment on bacteriophage and DNA thin filmsThe main goal of PUR experiment (phage and uracil response) is to examine and quantify the effect of specific space conditions on nucleic acid models. To achieve this an improved method was elaborated for the preparation of DNA and bacteriophage thin films. The homogeneity of the films was controlled by UV spectroscopy and microscopy. To provide experimental evidence for the hypothesis that interplanetary transfer of the genetic material is possible, phage T7 and isolated T7 DNA thin films have been exposed to selected space conditions: intense UVC radiation (lambda=254 nm) and high vacuum (10(-4) Pa). The effects of DNA hydration, conformation and packing on UV radiation damage were examined. Characteristic changes in the absorption spectrum, in the electrophoretic pattern of DNA and the decrease of the amount of PCR products have been detected indicating the photodamage of isolated and intraphage DNA. c2004 COSPAR. Published by Elsevier Ltd. All rights reserved.
Document ID
20050193988
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
Authors
Fekete, A.
(Institute of Biophysics and Radiation Biology, Semmelweis University Budapest, Hungary)
Ronto, Gy
Hegedus, M.
Modos, K.
Berces, A.
Kovacs, G.
Lammer, H.
Panitz, C.
Date Acquired
August 23, 2013
Publication Date
January 1, 2004
Publication Information
Publication: Advances in space research : the official journal of the Committee on Space Research (COSPAR)
Volume: 33
Issue: 8
Subject Category
Life Sciences (General)
Distribution Limits
Public
Copyright
Other

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