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G2 Chromatid Damage and Repair Kinetics in Normal Human Fibroblast Cells Exposed to Low-or High-LET RadiationRadiation-induced chromosome damage can be measured in interphase using the Premature Chromosome Condensation (PCC) technique. With the introduction of a new PCC technique using the potent phosphatase inhibitor calyculin-A, chromosomes can be condensed within five minutes, and it is now possible to examine the early damage induced by radiation. Using this method, it has been shown that high-LET radiation induces a higher frequency of chromatid breaks and a much higher frequency of isochromatid breaks than low-LET radiation. The kinetics of chromatid break rejoining consists of two exponential components representing a rapid and a slow time constant, which appears to be similar for low- and high- LET radiations. However, after high-LET radiation exposures, the rejoining process for isochromatid breaks influences the repair kinetics of chromatid-type breaks, and this plays an important role in the assessment of chromatid break rejoining in the G2 phase of the cell cycle.
Document ID
20060003825
Acquisition Source
Johnson Space Center
Document Type
Reprint (Version printed in journal)
Authors
Kawata, T.
(Chiba Univ. Chiba, Japan)
Ito, H.
(Chiba Univ. Chiba, Japan)
Uno, T.
(Chiba Univ. Chiba, Japan)
Saito, M.
(Chiba Univ. Chiba, Japan)
Yamamoto, S.
(Chiba Univ. Chiba, Japan)
Furusawa, Y.
(National Inst. of Radiological Sciences Chiba, Japan)
Durante, M.
(Naples II Univ. Naples, Italy)
George, K.
(NASA Johnson Space Center Houston, TX, United States)
Wu, H.
(NASA Johnson Space Center Houston, TX, United States)
Cucinotta, F. A.
(NASA Johnson Space Center Houston, TX, United States)
Date Acquired
August 23, 2013
Publication Date
January 1, 2004
Publication Information
Publication: Cytogenetic and Genome Research
Volume: 104
Subject Category
Life Sciences (General)
Report/Patent Number
JSC-CN-8695
Distribution Limits
Public
Copyright
Other

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