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Hybrid MD-Nernst Planck Model of Alpha-hemolysin Conductance PropertiesMotivated by experiments in which an applied electric field translocates polynucleotides through an alpha-hemolysin protein channel causing ionic current transient blockade, a hybrid simulation model is proposed to predict the conductance properties of the open channel. Time scales corresponding to ion permeation processes are reached using the Poisson-Nemst-Planck (PNP) electro-diffusion model in which both solvent and local ion concentrations are represented as a continuum. The diffusion coefficients of the ions (K(+) and Cl(-)) input in the PNP model are, however, calculated from all-atom molecular dynamics (MD). In the MD simulations, a reduced representation of the channel is used. The channel is solvated in a 1 M KCI solution, and an external electric field is applied. The pore specific diffusion coefficients for both ionic species are reduced 5-7 times in comparison to bulk values. Significant statistical variations (17-45%) of the pore-ions diffusivities are observed. Within the statistics, the ionic diffusivities remain invariable for a range of external applied voltages between 30 and 240mV. In the 2D-PNP calculations, the pore stem is approximated by a smooth cylinder of radius approx. 9A with two constriction blocks where the radius is reduced to approx. 6A. The electrostatic potential includes the contribution from the atomistic charges. The MD-PNP model shows that the atomic charges are responsible for the rectifying behaviour and for the slight anion selectivity of the a-hemolysin pore. Independent of the hierarchy between the anion and cation diffusivities, the anionic contribution to the total ionic current will dominate. The predictions of the MD-PNP model are in good agreement with experimental data and give confidence in the present approach of bridging time scales by combining a microscopic and macroscopic model.
Document ID
20060021485
Acquisition Source
Ames Research Center
Document Type
Reprint (Version printed in journal)
Authors
Cozmuta, Ioana
(Eloret Corp. Moffett Field, CA, United States)
O'Keefer, James T.
(NASA Ames Research Center Moffett Field, CA, United States)
Bose, Deepak
(Eloret Corp. Moffett Field, CA, United States)
Stolc, Viktor
(NASA Ames Research Center Moffett Field, CA, United States)
Date Acquired
August 23, 2013
Publication Date
February 15, 2006
Publication Information
Publication: Molecular Simulation
Publisher: Taylor and Francis Group Ltd.
Volume: 31
Issue: 3-Feb
ISSN: 0892-7022
Subject Category
Electronics And Electrical Engineering
Funding Number(s)
CONTRACT_GRANT: NAS2-99092
Distribution Limits
Public
Copyright
Other

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