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Fluorescent Approaches to High Throughput CrystallographyWe have shown that by covalently modifying a subpopulation, less than or equal to 1%, of a macromolecule with a fluorescent probe, the labeled material will add to a growing crystal as a microheterogeneous growth unit. Labeling procedures can be readily incorporated into the final stages of purification, and the presence of the probe at low concentrations does not affect the X-ray data quality or the crystallization behavior. The presence of the trace fluorescent label gives a number of advantages when used with high throughput crystallizations. The covalently attached probe will concentrate in the crystal relative to the solution, and under fluorescent illumination crystals show up as bright objects against a dark background. Non-protein structures, such as salt crystals, will not incorporate the probe and will not show up under fluorescent illumination. Brightly fluorescent crystals are readily found against less bright precipitated phases, which under white light illumination may obscure the crystals. Automated image analysis to find crystals should be greatly facilitated, without having to first define crystallization drop boundaries as the protein or protein structures is all that shows up. Fluorescence intensity is a faster search parameter, whether visually or by automated methods, than looking for crystalline features. We are now testing the use of high fluorescence intensity regions, in the absence of clear crystalline features or "hits", as a means for determining potential lead conditions. A working hypothesis is that kinetics leading to non-structured phases may overwhelm and trap more slowly formed ordered assemblies, which subsequently show up as regions of brighter fluorescence intensity. Preliminary experiments with test proteins have resulted in the extraction of a number of crystallization conditions from screening outcomes based solely on the presence of bright fluorescent regions. Subsequent experiments will test this approach using a wider range of proteins. The trace fluorescently labeled crystals will also emit with sufficient intensity to aid in the automation of crystal alignment using relatively low cost optics, further increasing throughput at synchrotrons.
Document ID
20060024734
Acquisition Source
Marshall Space Flight Center
Document Type
Conference Paper
Authors
Pusey, Marc L.
(NASA Marshall Space Flight Center Huntsville, AL, United States)
Forsythe, Elizabeth
(Nektar Therapeutics Huntsville, AL, United States)
Achari, Aniruddha
(Raytheon Co. Huntsville, AL, United States)
Date Acquired
August 23, 2013
Publication Date
January 1, 2006
Subject Category
Geophysics
Meeting Information
Meeting: 2006 PSI2 Botlenecks Workshop
Location: Bethesda, MD
Country: United States
Start Date: April 13, 2006
End Date: April 14, 2006
Sponsors: National Inst. of Health
Distribution Limits
Public
Copyright
Other

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