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Inter- and Intra-Chromosomal Aberrations in Human Cells Exposed in vitro to Space-like RadiationsEnergetic heavy ions pose a great health risk to astronauts in extended ISS and future exploration missions. High-LET heavy ions are particularly effective in causing various biological effects, including cell inactivation, genetic mutations and cancer induction. Most of these biological endpoints are closely related to chromosomal damage, which can be utilized as a biomarker for radiation insults. Previously, we had studied chromosome aberrations in human lymphocytes and fibroblasts induced by both low- and high-LET radiation using FISH and multicolor fluorescence in situ hybridization (mFISH) techniques. In this study, we exposed human cells in vitro to gamma rays and energetic particles of varying types and energies and dose rates, and analyzed chromosomal damages using the multicolor banding in situ hybridization (mBAND) procedure. Confluent human epithelial cells and lymphocytes were exposed to energetic heavy ions at NASA Space Radiation Laboratory (NSRL) at the Brookhaven National Laboratory (Upton, NY) or Cs-137 gamma radiation source at the Baylor College (Houston, TX). After colcemid and Calyculin A treatment, cells were fixed and painted with XCyte3 mBAND kit (MetaSystems) and chromosome aberrations were analyzed with mBAND analysis system (MetaSystems). With this technique, individually painted chromosomal bands on one chromosome allowed the identification of interchromosomal aberrations (translocation to unpainted chromosomes) and intrachromosomal aberrations (inversions and deletions within a single painted chromosome). The possible relationship between the frequency of inter- and intra-chromosomal exchanges and the track structure of radiation is discussed. The work was supported by the NASA Space Radiation Health Program.
Document ID
20070028912
Acquisition Source
Johnson Space Center
Document Type
Conference Paper
Authors
Hada, Megumi
(NASA Johnson Space Center Houston, TX, United States)
Cucinotta, F. A.
(NASA Johnson Space Center Houston, TX, United States)
Gonda, S. R.
(NASA Johnson Space Center Houston, TX, United States)
Wu, H.
(NASA Johnson Space Center Houston, TX, United States)
Date Acquired
August 23, 2013
Publication Date
November 16, 2005
Subject Category
Life Sciences (General)
Meeting Information
Meeting: The 1st Asian Congress of Radiation Research
Location: Hiroshima
Country: Japan
Start Date: November 16, 2005
End Date: November 17, 2005
Sponsors: Japanese Association for Radiation Research
Distribution Limits
Public
Copyright
Work of the US Gov. Public Use Permitted.

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