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Bench-Top Antigen Detection Technique that Utilizes Nanofiltration and Fluorescent Dyes which Emit and Absorb Light in the Near InfraredThis article discusses the development of a bench-top technique to detect antigens in fluids. The technique involves the use of near infrared NIR fluorescent dyes conjugated to antibodies, centrifugation, nanofilters, and spectrometry. The system used to detect the antigens utilizes a spectrometer, fiber optic cables, NIR laser, and laptop computer thus making it portable and ideally suited for desk top analysis. Using IgM as an antigen and the secondary antibody, anti-IgM conjugated to the near infrared dye, IRDye (trademark) 800, for detection, we show that nanofiltration can efficiently and specifically separate antibody-antigen complexes in solution and that the complexes can be detected by a spectrometer and software using NIR laser excitation at 778 nm and NIR dye offset emission at 804 nm. The peak power detected at 778 nm for the excitation emission and at 804 nm for the offset emission is 879 pW (-60.06 dBm) and 35.7 pW (-74.5 dBm), respectively.
Document ID
20080006068
Acquisition Source
Glenn Research Center
Document Type
Reprint (Version printed in journal)
Authors
Varaljay-Spence, Vanessa A.
(NASA Glenn Research Center Cleveland, OH, United States)
Scardelletti, Maximilian C.
(NASA Glenn Research Center Cleveland, OH, United States)
Date Acquired
August 24, 2013
Publication Date
September 13, 2007
Publication Information
Publication: Journal of Laser Applications
Publisher: Laser Inst. of America
Volume: 19
Issue: 4
ISSN: 0142-346X
Subject Category
Lasers And Masers
Funding Number(s)
WBS: WBS 122711.03.04.03.01
Distribution Limits
Public
Copyright
Other

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