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In vitro Catecholamine Exposure Produces Variable Effects on the B7 Costimulatory Pathway in Human Monocytic CellsCatecholamines have been associated with immunomodulation of the adaptive immune system towards a Th2 response in vitro. We therefore examined the role of in vitro epinephrine (EPI) and norepinephrine (NE) exposure on the B7 costimulatory expression of antigen presenting cells (APC) from human monocytic cell lines and human peripheral blood mononuclear cells (PBMC). THP1 monocytic cells and CD14+ cells from normal human PBMC were stimulated with lipopolysaccharide (LPS) and incubated with physiologic stress levels (10(exp -6) - 10(exp -8)M) of EPI or NE for 24 hours. Cells were subsequently stained with CD80 FITC, CD86 PE, and CD14 PC5 antibodies and analyzed by flow cytometry for changes in fluorescence and mean fluorescence intensity (MFI). Exposure of THP1 to EPI in vitro at concentrations of 10(exp -6), 10(exp -7) and 10(exp -8)M significantly decreased mean CD80 from 42 plus or minus 0.7% to 11 plus or minus 0.44%, 19.1 plus or minus 2.0%, and 30.7 plus or minus 2.1% expression, respectively (p less than 0.01). In addition, CD86 expression increased with EPI at 10(exp -6), 10(exp -7) and 10(exp -8) M from 9.2 plus or minus 0.52% to 41 plus or minus 3.8%, 26.4 plus or minus 1.9%, and 15.74 plus or minus 1.8% expression, respectively (p less than 0.01). Similar results for mean CD80 and CD86 percent expression were observed for CD14+ cells from PBMC with a sample size of N = 6 and for NE when substituted for EPI. The data show that in vitro exposure to catecholamines significantly decreases %CD86 expression and significantly increases %CD86 expression in THP1 cells and human CD14+ APC. Previous studies have suggested an association between increased CD86 expression and TH2 activity. Thus, these data suggest that immunomodulation by catecholamines results in part by the variable effects of the B7 costimulatory pathway in APC.
Document ID
20080030930
Acquisition Source
Johnson Space Center
Document Type
Conference Paper
Authors
Salicru, A. N.
(Texas Univ. Health Science Center Houston, TX, United States)
Crucian, B.
(Wyle Labs., Inc. Houston, TX, United States)
Sams, Clarence
(NASA Johnson Space Center Houston, TX, United States)
Actor, J. K.
(Texas Univ. Health Science Center Houston, TX, United States)
Marshall, G. D., Jr.
(Mississippi Univ. Medical Center Jackson, MS, United States)
Date Acquired
August 24, 2013
Publication Date
May 12, 2006
Subject Category
Life Sciences (General)
Meeting Information
Meeting: Immunology 2006
Location: Boston, MA
Country: United States
Start Date: May 12, 2006
End Date: May 16, 2006
Sponsors: American Association of Immunologists
Distribution Limits
Public
Copyright
Other

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