Record Details

Record 1 of 1
Microbial Monitoring of Common Opportunistic Pathogens by Comparing Multiple Real-Time PCR Platforms for Potential Space Applications
NTRS Full-Text: Click to View  [PDF Size: 3.10 MB]
Author and Affiliation:
Oubre, Cherie M.(Wyle Labs., Inc., Houston, TX, United States);
Birmele, Michele N.(Sierra Lobo, Inc., Kennedy Space Center, FL, United States);
Castro, Victoria A.(Enterprise Advisory Services, Inc., Houston, TX, United States);
Venkateswaran, Kasthuri J.(Jet Propulsion Lab., California Inst. of Tech., Pasadena, CA, United States);
Vaishampayan, Parag A.(Jet Propulsion Lab., California Inst. of Tech., Pasadena, CA, United States);
Jones, Kathy U.(NASA Marshall Space Flight Center, Huntsville, AL, United States);
Singhal, Adesh(NASA Marshall Space Flight Center, Huntsville, AL, United States);
Johnston, Angela S.(NASA Marshall Space Flight Center, Huntsville, AL, United States);
Roman, Monserrate C.(NASA Marshall Space Flight Center, Huntsville, AL, United States);
Ozbolt, Tamra A.(Emerald City Initiatives, Inc., Grant, AL, United States);
Jett, Daniel X.(Teledyne Brown Engineering, Huntsville, AL, United States);
Roberts, Michael S.(CSS-Dynamac, Kennedy Space Center, FL, United States);
Ott, C. Mark(NASA Johnson Space Center, Houston, TX, United States)
Abstract: Because the International Space Station is a closed environment with rotations of astronauts and equipment that each introduce their own microbial flora, it is necessary to monitor the air, surfaces, and water for microbial contamination. Current microbial monitoring includes labor- and time-intensive methods to enumerate total bacterial and fungal cells, with limited characterization, during in-flight testing. Although this culture-based method is sufficient for monitoring the International Space Station, on future long-duration missions more detailed characterization will need to be performed during flight, as sample return and ground characterization may not be available. At a workshop held in 2011 at NASA's Johnson Space Center to discuss alternative methodologies and technologies suitable for microbial monitoring for these long-term exploration missions, molecular-based methodologies such as polymerase chain reaction (PCR) were recommended. In response, a multi-center (Marshall Space Flight Center, Johnson Space Center, Jet Propulsion Laboratory, and Kennedy Space Center) collaborative research effort was initiated to explore novel commercial-off-the-shelf hardware options for space flight environmental monitoring. The goal was to evaluate quantitative or semi-quantitative PCR approaches for low-cost in-flight rapid identification of microorganisms that could affect crew safety. The initial phase of this project identified commercially available platforms that could be minimally modified to perform nominally in microgravity. This phase was followed by proof-of-concept testing of the highest qualifying candidates with a universally available challenge organism, Salmonella enterica. The analysis identified two technologies that were able to perform sample-to-answer testing with initial cell sample concentrations between 50 and 400 cells. In addition, the commercial systems were evaluated for initial flight safety and readiness.
Publication Date: Jan 01, 2013
Document ID:
20130013657
(Acquired Jun 13, 2013)
Subject Category: MAN/SYSTEM TECHNOLOGY AND LIFE SUPPORT
Report/Patent Number: KSC-2013-084
Document Type: Conference Paper
Meeting Information: 43rd International Conference on Environmental Systems; 14-18 Jul. 2013; Vail, CO; United States
Meeting Sponsor: American Inst. of Aeronautics and Astronautics; Reston, VA, United States
Contract/Grant/Task Num: NNK11EA08C
Financial Sponsor: NASA Kennedy Space Center; Cocoa Beach, FL, United States
Organization Source: Wyle Labs., Inc.; Houston, TX, United States
Description: 12p; In English; Original contains black and white illustrations
Distribution Limits: Unclassified; Publicly available; Unlimited
Rights: Copyright; Distribution as joint owner in the copyright
NASA Terms: CLOSED ECOLOGICAL SYSTEMS; ENVIRONMENTAL MONITORING; LONG DURATION SPACE FLIGHT; SPACECRAFT; SPACECRAFT CABIN ATMOSPHERES; SALMONELLA; SPACE LABORATORIES; MICROORGANISMS; PATHOGENS; BACTERIA; POLYMERASE CHAIN REACTION; REAL TIME OPERATION; TECHNOLOGY UTILIZATION; COMMERCIAL OFF-THE-SHELF PRODUCTS; PROVING
› Back to Top
 
NASA Logo, External Link

NASA Official: Gerald Steeman
Sponsored By: NASA Scientific and Technical Information Program
Site Curator: STI Support Services
Last Modified: June 13, 2013

Privacy Policy & Important Notices Disclaimers, Copyright, Terms of Use Freedom of Information Act USA.gov NASA.gov NASA OCIO Free Adobe PDF Reader Free MS Word Viewer