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mBAND Analysis of Early and Late Damages in the Chromosome of Human Lymphocytes after Exposures to Gamma Rays and Fe Ions
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Author and Affiliation:
Sunagawa, Mayumi(Texas Southern Univ., Houston, TX, United States);
Zhang, Ye(Wyle Labs., Inc., Houston, TX, United States);
Yeshitla, Samrawit(Texas Southern Univ., Houston, TX, United States);
Kadhim, Munira(Oxford Brookes Univ., Headington, United Kingdom);
Wilson, Bobby(Texas Southern Univ., Houston, TX, United States);
Wu, Honglu(NASA Johnson Space Center, Houston, TX, United States)
Abstract: Stable type chromosome aberrations that survive multiple generations of cell division include translocation and inversions. An efficient method to detect an inversion is multi-color banding fluorescent in situ hybridization (mBAND) which allows identification of both inter- and intrachromosome aberrations simultaneously. Post irradiation, chromosome aberrations may also arise after multiple cell divisions as a result of genomic instability. To investigate the stable or late-arising chromosome aberrations induced after radiation exposure, we exposed human lymphocytes to gamma rays and Fe ions ex vivo, and cultured the cells for multiple generations. Chromosome aberrations were analyzed in cells collected at first mitosis and at several time intervals during the culture period post irradiation. With gamma irradiation, about half of the damages observed at first mitosis remained after 7 day- and 14 day- culture, suggesting the transmissibility of damages to the surviving progeny. At the doses that produced similar frequencies of gamma-induced chromosome aberrations as observed at first mitosis, a significantly lower yield of aberrations remained at the same population doublings after Fe ion exposure. At these equitoxic doses, more complex type aberrations were observed for Fe ions, indicating that Fe ion-induced initial chromosome damages are more severe and may lead to cell death. Detailed analysis of breaks participating in total chromosome exchanges within the first cell cycle post irradiation revealed a common hotspot located in the 3p21 region, which is a known fragile site corresponding to the band 6 in the mBand analysis. The breakpoint distribution in chromosomes collected at 7 days, but not at 14 days, post irradiation appeared similar to the distribution in cells collected within the first cell cycle post irradiation. The breakpoint distribution for human lymphocytes after radiation exposure was different from the previously published distribution for human mammary epithelial cells, indicating that interphase chromatin folding structures play a role in the distribution of radiation-induced breaks.
Publication Date: May 15, 2013
Document ID:
20130013733
(Acquired Jun 24, 2013)
Subject Category: AEROSPACE MEDICINE
Report/Patent Number: JSC-CN-28822
Document Type: Conference Paper
Meeting Information: HITSRS2013. Hyeavy Ion in Therapy and Space Radiation Symposium 2013; 15-18 May 2013; Chiba; Japan
Financial Sponsor: NASA Johnson Space Center; Houston, TX, United States
Organization Source: NASA Johnson Space Center; Houston, TX, United States
Description: 1p; In English; Original contains color illustrations
Distribution Limits: Unclassified; Publicly available; Unlimited
Rights: Copyright; Distribution as joint owner in the copyright
NASA Terms: CELL DIVISION; CELLS (BIOLOGY); CHROMOSOME ABERRATIONS; DEOXYRIBONUCLEIC ACID; GAMMA RAYS; HUMAN BEINGS; IRON ISOTOPES; LYMPHOCYTES; MITOSIS; RADIATION DOSAGE; RADIATION EFFECTS
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