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Microbial Burden Approach : New Monitoring Approach for Measuring Microbial BurdenAdvantages of new approach for differentiating live cells/ spores from dead cells/spores. Four examples of Salmonella outbreaks leading to costly destruction of dairy products. List of possible collaboration activities between JPL and other industries (for future discussion). Limitations of traditional microbial monitoring approaches. Introduction to new approach for rapid measurement of viable (live) bacterial cells/spores and its areas of application. Detailed example for determining live spores using new approach (similar procedure for determining live cells). JPL has developed a patented approach for measuring amount of live and dead cells/spores. This novel "molecular" method takes less than 5 to 7 hrs. compared to the seven days required using conventional techniques. Conventional "molecular" techniques can not discriminate live cells/spores among dead cells/spores. The JPL-developed novel method eliminates false positive results obtained from conventional "molecular" techniques that lead to unnecessary delay in the processing and to unnecessary destruction of food products.
Document ID
20140002082
Acquisition Source
Jet Propulsion Laboratory
Document Type
Presentation
External Source(s)
Authors
Venkateswaran, Kasthuri
(Jet Propulsion Lab., California Inst. of Tech. Pasadena, CA, United States)
Vaishampayan, Parag
(Jet Propulsion Lab., California Inst. of Tech. Pasadena, CA, United States)
Barmatz, Martin
(Jet Propulsion Lab., California Inst. of Tech. Pasadena, CA, United States)
Date Acquired
March 19, 2014
Publication Date
February 1, 2013
Subject Category
Quality Assurance And Reliability
Life Sciences (General)
Distribution Limits
Public
Copyright
Other
Keywords
microbial monitoring
biosensor detection
Propidium monoazide (PMA)
decontamination system

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