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Measuring Total and Germinable Spore PopulationsIt has been shown that bacterial endospores can be enumerated using a microscopy based assay that images the luminescent halos from terbium ions bound to dipicolinic acid, a spore specific chemical marker released upon spore germination. Further development of the instrument has simplified it towards automation while at the same time improving image quality. Enumeration of total spore populations has also been developed allowing measurement of the percentage of viable spores in any population by comparing the germinable/culturable spores to the total. Percentage viability will allow a more quantitative comparison of the ability of spores to survive across a wide range of extreme environments.
Document ID
20150006996
Acquisition Source
Jet Propulsion Laboratory
Document Type
Conference Paper
External Source(s)
Authors
Noell, A.C.
(Jet Propulsion Lab., California Inst. of Tech. Pasadena, CA, United States)
Yung, P.T.
(Chinese Univ. of Hong Kong Kowloon, Hong Kong)
Yang, W.
(Jet Propulsion Lab., California Inst. of Tech. Pasadena, CA, United States)
Lee, C.
(Jet Propulsion Lab., California Inst. of Tech. Pasadena, CA, United States)
Ponce, A.
(Jet Propulsion Lab., California Inst. of Tech. Pasadena, CA, United States)
Date Acquired
April 30, 2015
Publication Date
October 2, 2011
Subject Category
Exobiology
Meeting Information
Meeting: EPSC-DPS Joint Meeting 2011
Location: Nantes
Country: France
Start Date: October 2, 2011
End Date: October 7, 2011
Sponsors: European Planetary Science Congress, American Astronomical Society
Distribution Limits
Public
Copyright
Other
Keywords
microbial life
environmental sample results
spore enumeration

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