Record Details

Altered Gravity Induces Oxidative Stress in Drosophila Melanogaster
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Author and Affiliation:
Bhattacharya, Sharmila(NASA Ames Research Center, Moffett Field, CA United States);
Hosamani, Ravikumar(Oak Ridge Associated Universities, Moffett Field, CA, United States)
Abstract: Altered gravity environments can induce increased oxidative stress in biological systems. Microarray data from our previous spaceflight experiment (FIT experiment on STS-121) indicated significant changes in the expression of oxidative stress genes in adult fruit flies after spaceflight. Currently, our lab is focused on elucidating the role of hypergravity-induced oxidative stress and its impact on the nervous system in Drosophila melanogaster. Biochemical, molecular, and genetic approaches were combined to study this effect on the ground. Adult flies (2-3 days old) exposed to acute hypergravity (3g, for 1 hour and 2 hours) showed significantly elevated levels of Reactive Oxygen Species (ROS) in fly brains compared to control samples. This data was supported by significant changes in mRNA expression of specific oxidative stress and antioxidant defense related genes. As anticipated, a stress-resistant mutant line, Indy302, was less vulnerable to hypergravity-induced oxidative stress compared to wild-type flies. Survival curves were generated to study the combined effect of hypergravity and pro-oxidant treatment. Interestingly, many of the oxidative stress changes that were measured in flies showed sex specific differences. Collectively, our data demonstrate that altered gravity significantly induces oxidative stress in Drosophila, and that one of the organs where this effect is evident is the brain.
Publication Date: Nov 10, 2015
Document ID:
20160001641
(Acquired Feb 08, 2016)
Subject Category: LIFE SCIENCES (GENERAL); EXOBIOLOGY
Report/Patent Number: ARC-E-DAA-TN27793
Document Type: Oral/Visual Presentation
Meeting Information: Annual Meeting of the American Society for Gravitational and Space Research ; 31st ; 10-14 Nov. 2015; Alexandria, VA; United States
Meeting Sponsor: American Society for Gravitational and Space Research; Bristow, VA, United States
Contract/Grant/Task Num: NNH06CC03B
Financial Sponsor: NASA Ames Research Center; Moffett Field, CA United States
Organization Source: NASA Ames Research Center; Moffett Field, CA United States
Description: 10p; In English
Distribution Limits: Unclassified; Publicly available; Unlimited
Rights: Copyright; Distribution as joint owner in the copyright
NASA Terms: HIGH GRAVITY ENVIRONMENTS; ANTIOXIDANTS; STRESS (PHYSIOLOGY); GENES; DROSOPHILA; BRAIN; SIGNIFICANCE; SPACE FLIGHT; REACTIVITY; SURVIVAL; SEX FACTOR; PEROXIDES; GLUTATHIONE
Other Descriptors: OXIDATIVE STRESS; DROSOPHILA MELANOGASTER; REACTIVE OXYGEN SPECIES
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