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Non-conventional approaches to food processing in CELSS, 1. Algal proteins: Characterization and process optimizationProtein isolate obtained from green algae cultivated under controlled conditions was characterized. Molecular weight determination of fractionated algal proteins using SDS-polyacrylamide gel electrophoresis revealed a wide spectrum of molecular weights ranging from 15,000 to 220,000. Isoelectric points of dissociated proteins were in the range of 3.95 to 6.20. Amino acid composition of protein isolate compared favorably with FAO standards. High content of essential amino acids leucine, valine, phenylalanine and lysine make algal protein isolate a high quality component of closed ecological life support system diets. To optimize the removal of algal lipids and pigments supercritical carbon dioxide extraction (with and without ethanol as a co-solvent) was used. Addition of ethanol to supercritical carbon dioxide resulted in more efficient removal of algal lipids and produced protein isolate with a good yield and protein recovery. The protein isolate extracted by the above mixture had an improved water solubility.
Document ID
Document Type
Conference Paper
Nakhost, Z.
(Massachusetts Inst. of Tech. Cambridge., United States)
Karel, M.
(Massachusetts Inst. of Tech. Cambridge., United States)
Krukonis, V. J.
(Phasex Corp. Lawrence, Mass., United States)
Date Acquired
September 5, 2013
Publication Date
September 1, 1987
Publication Information
Publication: NASA, Ames Research Center, Controlled Ecological Life Support System: Regenerative Life Support Systems in Space
Subject Category
Man/System Technology And Life Support
Distribution Limits
Work of the US Gov. Public Use Permitted.

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