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NASA's Rodent Research Project: Validation of Capabilities for Conducting Long Duration Experiments in SpaceResearch using rodents is an essential tool for advancing biomedical research on Earth and in space. Prior rodent experiments on the Shuttle were limited by the short flight duration. The International Space Station (ISS) provides a new platform for conducting rodent experiments under long duration conditions. Rodent Research (RR)-1 was conducted to validate flight hardware, operations, and science capabilities that were developed at the NASA Ames Research Center. Twenty C57BL6J adult female mice were launched on Sept 21, 2014 in a Dragon Capsule (SpaceX-4), then transferred to the ISS for a total time of 21-22 days (10 commercial mice) or 37 days (10 validation mice). Tissues collected on-orbit were either rapidly frozen or preserved in RNAlater at -80C (n2group) until their return to Earth. Remaining carcasses on-orbit were rapidly frozen for dissection post-flight. The three controls groups at Kennedy Space Center consisted of: Basal mice euthanized at the time of launch, Vivarium controls housed in standard cages, and Ground Controls (GC) housed in flight hardware within an environmental chamber. Upon return to Earth, there were no differences in body weights between Flight (FLT) and GC at the end of the 37 days in space. Liver enzyme activity levels of FLT mice and all control mice were similar in magnitude to those of the samples that were processed under optimal conditions in the laboratory. Liver samples dissected on-orbit yielded high quality RNA (RIN8.99+-0.59, n7). Liver samples dissected post-flight from the intact, frozen FLT carcasses yielded RIN of 7.27 +- 0.52 (n6). Additionally, wet weights of various tissues were measured. Adrenal glands and spleen showed no significant differences in FLT compared to GC although thymus and livers weights were significantly greater in FLT compared to GC. Over 3,000 tissue aliquots collected post-flight from the four groups of mice were deposited into the Ames Life Science Data Archives for future Biospecimen Sharing Program. Together, the RR validation flight successfully demonstrates the capability to support long-duration experimentation on the ISS to achieve both basic science and biomedical objectives.
Document ID
20150023125
Document Type
Presentation
Authors
Choi, Sungshin Y. (Wyle Labs., Inc. Houston, TX, United States)
Cole, Nicolas (Logyx, LLC Mountain View, CA, United States)
Reyes, America (Wyle Labs., Inc. Houston, TX, United States)
Lai, San-Huei (Wyle Labs., Inc. Houston, TX, United States)
Klotz, Rebecca (Wyle Labs., Inc. Houston, TX, United States)
Beegle, Janet E. (NASA Ames Research Center Moffett Field, CA United States)
Wigley, Cecilia L. (NASA Ames Research Center Moffett Field, CA United States)
Pletcher, David (NASA Ames Research Center Moffett Field, CA United States)
Globus, Ruth K. (NASA Ames Research Center Moffett Field, CA United States)
Date Acquired
December 15, 2015
Publication Date
November 14, 2015
Subject Category
Life Sciences (General)
Report/Patent Number
ARC-E-DAA-TN27932
Meeting Information
Annual Meeting of ASGSR(Alexandria, VA)
Funding Number(s)
CONTRACT_GRANT: NNA14AB82C
Distribution Limits
Public
Copyright
Public Use Permitted.
Keywords
spaceflight
rodents
ISS

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NameType 20150023125.pdf STI

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