The BioSentinel Bioanalytical Microsystem: Characterizing DNA Radiation Damage in Living Organisms Beyond Earth Orbit

Ricco, A. J.; Hanel, R.; Bhattacharya, S.; Boone, T.; Tan, M.; Mousavi, A.; Rademacher, A.; Schooley, A.; Klamm, B.; Benton, J.; Padgen, M.; Gentry, D.; Friedericks, C.; Defouw, G.; Parra, M.; Santa Maria, S.; Marina, D.; Swan, B. G.; Wheeler, S.; Gavalas, S.; Lewis, B.; Sanchez, H.; Chartres, J.; Lusby, T.

We will present details and initial lab test results from an integrated bioanalytical microsystem designed to conduct the first biology experiments beyond low Earth orbit (LEO) since Apollo 17 (1972). The 14-kg, 12x24x37-cm BioSentinel spacecraft (Figure 1) assays radiation-responsive yeast in its science payload by measuring DNA double-strand breaks (DSBs) repaired via homologous recombination, a mechanism common to all eukaryotes including humans. S. cerevisiae (brewer's yeast) in 288 microwells are provided with nutrient and optically assayed for growth and metabolism via 3-color absorptimetry monthly during the 18-month mission. BioSentinel is one of several secondary payloads to be deployed by NASA's Exploration Mission 1 (EM-1) launch vehicle into approximately 0.95 AU heliocentric orbit in July 2018; it will communicate with Earth from up to 100 million km.

Document ID

20160007343

Document Type

Conference Paper

Authors

Ricco, A. J. (Dublin City Univ. Dublin, Ireland)

Hanel, R. (NASA Ames Research Center Moffett Field, CA United States)

Bhattacharya, S. (NASA Ames Research Center Moffett Field, CA United States)

Boone, T. (Millennium Engineering and Integration Co. Moffett Field, CA, United States)

Tan, M. (Millennium Engineering and Integration Co. Moffett Field, CA, United States)

Mousavi, A. (Stanford Univ. Stanford, CA, United States)

Rademacher, A. (Millennium Engineering and Integration Co. Moffett Field, CA, United States)

Schooley, A. (Millennium Engineering and Integration Co. Moffett Field, CA, United States)

Klamm, B. (NASA Ames Research Center Moffett Field, CA United States)

Benton, J. (Wyle Labs., Inc. Moffett Field, CA, United States)

Padgen, M. (Millennium Engineering and Integration Co. Moffett Field, CA, United States)

Gentry, D. (NASA Ames Research Center Moffett Field, CA United States)

Friedericks, C. (NASA Ames Research Center Moffett Field, CA United States)

Defouw, G. (SGT, Inc. Moffett Field, CA, United States)

Parra, M. (Wyle Labs., Inc. Moffett Field, CA, United States)

Santa Maria, S. (Wyle Labs., Inc. Moffett Field, CA, United States)

Marina, D. (Wyle Labs., Inc. Moffett Field, CA, United States)

Swan, B. G. (NASA Johnson Space Center Houston, TX, United States)

Wheeler, S. (NASA Johnson Space Center Houston, TX, United States)

Gavalas, S. (NASA Johnson Space Center Houston, TX, United States)

Lewis, B. (NASA Ames Research Center Moffett Field, CA United States)

Sanchez, H. (NASA Ames Research Center Moffett Field, CA United States)

Chartres, J. (Millennium Engineering and Integration Co. Moffett Field, CA, United States)

Lusby, T. (Millennium Engineering and Integration Co. Moffett Field, CA, United States)

Date Acquired

June 8, 2016

Publication Date

June 5, 2016

Subject Category

Report/Patent Number

Meeting Information

Hilton Head Workshop 2016: A Solid State Sensors, Actuators, and Microsystems Workshop(Hilton Head Island, SC)

Funding Number(s)

Distribution Limits

Public

Public Use Permitted.

Keywords

Available Downloads

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Type

20160007343.pdf

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NTRS

NTRS - NASA Technical Reports Server

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