Effects of inhalation of Freon 113 on laboratory animals

Four monkeys, 8 dogs, 40 mice, and 50 rats were exposed continuously to 2000 ppm Freon 113 in a Thomas Dome for 14 days. This exposure produced no mortalities nor adverse symptomology. There were no significant alterations in hematological values, clinical chemistries, electroencephalographic findings, body weights, or organ to body weight ratios. The effect of 2% Freon 113 on nicotinic transmission through the stellate ganglion of the spinal dog was also evaluated. The exposure induced a reduction in nicotinic transmission comparable to 2% halothane.