Increase of cold tolerance in cotton plant (Gossypium hirsutum L.) by mepiquat chloride

Three mepiquat chloride (MC) concentrations - 40, 70, and 100 g a.i./ha - were used to spray cotton (Gossypium hirsutum L., cultival McNair 220) plants to determine whether or not MC would increase their cold tolerance. Seven to ten days after the spray, the plants were exposed to three different cold treatments. No important difference in cold damage was noticed between the control and the MC-treated plants when they were exposed repeatedly to 4.5 C. No plants died when exposed to 0.5 C for 12 h; however, 90% of the 1st and 2nd leaves of the control plants were damaged. This was three times more damage than those leaves of plants treated with 70 and 100 g a.i./ha MC concentrations; 60% f the control and 10-20% of the MC-treated plants died when the plants were subjected to a cold hardening process with 15.5 C day (12 h) and 1.7 C night (12 h) for 10 days, and then, held at -2.2 C for 24 hours. The electrolyte leakage and reflectance measurement data showed that the cell membranes of the MC-treated plants sustained much less damage than those of the control. Freezing injury was easily assessed by reflectance measurements at the 1.65 micrometer wavelength.