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Effects of Simulated Weightlessness on Mammalian Development. Part 2: Meiotic Maturation of Mouse Oocytes During Clinostat RotationIn order to understand the role of gravity in basic cellular processes that are important during development, the effects of a simulated microgravity environment on mammalian gametes and early embryos cultured in vitro are examined. A microgravity environment is simulated by use of a clinostat, which essentially reorients cells relative to the gravity vector. Initial studies have focused on assessing the effects of clinostat rotation on the meiotic progression of mouse oocytes. Modifications centered on providing the unique in vitro culture of the clinostat requirements of mammalian oocytes and embryos: 37 C temperature, constant humidity, and a 5% CO2 in air environment. The oocytes are observed under the dissecting microscope for polar body formation and gross morphological appearance. They are then processed for cytogenetic analysis.
Document ID
19850017783
Acquisition Source
Legacy CDMS
Document Type
Conference Paper
Authors
Wolgemuth, D. J.
(Columbia Univ. New York, NY, United States)
Grills, G. S.
(Columbia Univ. New York, NY, United States)
Date Acquired
August 12, 2013
Publication Date
April 1, 1985
Publication Information
Publication: NASA. Washington NASA Space Biol. Program:
Subject Category
Life Sciences (General)
Accession Number
85N26094
Funding Number(s)
CONTRACT_GRANT: NAGW-346
Distribution Limits
Public
Copyright
Work of the US Gov. Public Use Permitted.
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