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Fusion proteins as alternate crystallization paths to difficult structure problemsThe three-dimensional structure of a peptide fusion product with glutathione transferase from Schistosoma japonicum (SjGST) has been solved by crystallographic methods to 2.5 A resolution. Peptides or proteins can be fused to SjGST and expressed in a plasmid for rapid synthesis in Escherichia coli. Fusion proteins created by this commercial method can be purified rapidly by chromatography on immobilized glutathione. The potential utility of using SjGST fusion proteins as alternate paths to the crystallization and structure determination of proteins is demonstrated.
Document ID
19950018111
Acquisition Source
Legacy CDMS
Document Type
Technical Memorandum (TM)
Authors
Carter, Daniel C. (NASA Marshall Space Flight Center Huntsville, AL, United States)
Rueker, Florian (Bodenkultur Univ. Vienna, Austria)
Ho, Joseph X. (NASA Marshall Space Flight Center Huntsville, AL, United States)
Lim, Kap (NASA Marshall Space Flight Center Huntsville, AL, United States)
Keeling, Kim (NASA Marshall Space Flight Center Huntsville, AL, United States)
Gilliland, Gary (Center for Advanced Research in Biotechnology Rockville, MD., United States)
Ji, Xinhua (Center for Advanced Research in Biotechnology Rockville, MD., United States)
Date Acquired
September 6, 2013
Publication Date
January 1, 1994
Subject Category
Life Sciences (General)
Report/Patent Number
NAS 1.15:110497
NASA-TM-110497
Report Number: NAS 1.15:110497
Report Number: NASA-TM-110497
Accession Number
95N24531
Distribution Limits
Public
Copyright
Work of the US Gov. Public Use Permitted.

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