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Regional differences in lectin binding patterns of vestibular hair cellsSurface glycoconjugates of hair cells and supporting cells in the vestibular endorgans of the bullfrog were identified using biotinylated lectins with different carbohydrate specificities. Lectin binding in hair cells was consistent with the presence of glucose and mannose (CON A), galactose (RCA-I), N-acetylgalactosamine (VVA), but not fucose (UEA-I) residues. Hair cells in the bullfrog sacculus, unlike those in the utriculus and semicircular canals, did not stain for N-acetylglucosamine (WGA) or N-acetylgalactosamine (VVA). By contrast, WGA and, to a lesser extent, VVA, differentially stained utricular and semicircular canal hair cells, labeling hair cells located in peripheral, but not central, regions. In mammals, WGA uniformly labeled Type 1 hair cells while labeling, as in the bullfrog, Type 2 hair cells only in peripheral regions. These regional variations were retained after enzymatic digestion. We conclude that vestibular hair cells differ in their surface glycoconjugates and that differences in lectin binding patterns can be used to identify hair cell types and to infer the epithelial origin of isolated vestibular hair cells.
Document ID
19950020308
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
Authors
Baird, Richard A.
(Legacy Good Samaritan Hospital Portland, OR, United States)
Schuff, N. R.
(Legacy Good Samaritan Hospital Portland, OR, United States)
Bancroft, J.
(Legacy Good Samaritan Hospital Portland, OR, United States)
Date Acquired
September 6, 2013
Publication Date
December 31, 1994
Publication Information
Publication: Comparative Transduction Mechanisms of Vestibular Otolith Hair Cells
ISSN: 0378-5955
Subject Category
Life Sciences (General)
Accession Number
95N26728
Distribution Limits
Public
Copyright
Work of the US Gov. Public Use Permitted.
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