Fluid Dynamic Verification Experiments on STS-70

Fluid dynamic experiments were flown on STS-70 as phase two of the engineering evaluation of the first bioreactor Engineering Development Unit (EDU#1). The phase one experiments were comparative cell cultures in identical units on earth and onboard STS-70. In phase two, two types of fluid dynamic experiments were performed. Qualitative comparisons of the basic flow patterns were evaluated with the use of 'dye' streaklines formed from alternate injections of either a mild acid or base solution into the external flow loop that was then perfused into the vessel. The presence of Bromothymol Blue in the fluid then caused color changes from yellow to blue or vice versa, indicating the basic fluid motions. This reversible change could be repeated as desired. In the absence of significant density differences in the fluid, the flow patterns in space should be the same as on earth. Video tape records of the flow patterns for a wide range of operating conditions were obtained. The second type of fluid dynamic experiment was the quantitative evaluation of the trajectories of solid beads of various densities and sizes. The beads were introduced into the vessel and the paths recorded on video tape, with the vessel operated at various rotation rates and flow perfusion rates. Because of space limitations, the video camera was placed as close as possible to the vessel, resulting in significant optical distortion. This report describes the analysis methods to obtain comparisons between the in-flight fluid dynamics and numerical models of the flow field. The methods include optical corrections to the video images and calculation of the bead trajectories for given operating conditions and initial bead locations.