Galaptin Mediates the Effect of Hypergravity on Vascular Smooth Muscle cell (SMC) Adhesion to Laminin Containing MatricesGalaptin, an endogenous beta-galactoside specific lectin, has been reported to bind to laminin and subsequently decrease the binding of SMC. Cellular function depend on cell:matrix interactions. Hypergravity (HGrav) affect a number of cellular functions, yet little is known about its affect on cell adhesion. We examined the possibility that galaptin mediates the effects of hypergravity on SMC adherence. Confluent primate aorta SMC cultures were subjected to Hgrav (centrifuged at 6G) for 24 and 48 hr. Cells were non-enzymatically dispersed, pretreated with antisense (AS-oligo) or control sense (SS-oligo) oligonucleotides to galaptin mRNA (0.01 micro g/ml), then seeded in uncoated or ECL-matrix coated plates. Adhesion of cells were monitored after 6 hr. HGrav increased adhesion by 100-300% compared to controls. AS-oligo decreased adhesion for both HGrav and control cells. SS-oligo did not affect adhesion for either HGrav or control cells. These studies show that HGrav affects cell adhesion and that galaptin expression is required for this effect.
Enahora, Fatisha T. (Clark-Atlanta Univ. GA United States)
Bosah, Francis N. (Clark-Atlanta Univ. GA United States)
Harris-Hooker, Sandra (Morehouse School of Medicine Atlanta, GA United States)
Sanford, Gary L. (Morehouse School of Medicine Atlanta, GA United States)
August 17, 2013
January 1, 1997
Publication: The First National Student Conference: NASA University Research Centers at Minority Institutions