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Preparation and Fluorescence Anisotropy Study of a Ribonuclease-Lucifer Yellow ConjugateWe have prepared a chemical derivative of ribonuclease A (RNase) with lucifer yellow (LY). The rotational dynamics of the LY-RNase conjugate were characterized by steady state and time resolved fluorescence techniques. Steady state anisotropy measurements were performed at varying viscosities at 10C and 20C, and the rotational correlation time of both RNase and the covalently linked LY probe were determined by time resolved frequency domain measurements. Our data suggest that the fluorophore is rigidly bound at 10C.
Document ID
20000105165
Acquisition Source
Marshall Space Flight Center
Document Type
Abstract
Authors
Malone, C. C. (NASA Marshall Space Flight Center Huntsville, AL United States)
Sumida, J. (NASA Marshall Space Flight Center Huntsville, AL United States)
Pusey, M. L. (NASA Marshall Space Flight Center Huntsville, AL United States)
Rose, M. Franklin
Date Acquired
August 19, 2013
Publication Date
January 1, 2000
Subject Category
Inorganic, Organic And Physical Chemistry
Distribution Limits
Public
Copyright
Work of the US Gov. Public Use Permitted.

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