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Differentiation and Genomic Instability in a Human Mammary Cell ModelHarvest of prophylactic mastectomy specimens from an obligate heterozygote for ataxia-telangiectasia provided autologous fibroblasts as well epithelial cells (HMEC). The routine availability of these autologous cells has provided an opportunity to study cell-cell interactions in coculture and monoculture, and in 3-dimensional cultures grown in the NASA rotating bioreactor. HMEC and stromal fibroblasts grown in 2-dimensional monoculture were both observed to produce extracellular matrix. Similar matrix was encountered in 3-dimensional cultures containing HMEC. Metaphases were analyzed. For stromal fibroblasts, genomic aberrations were found in 18% of metaphase spreads. For HMEC, aberrations were greater such that a majority were found to be abnormal. The level of genomic instability determined for these noncancerous cells in 2-dimensional monoculture should be useful for generating a human cell model that can correlate the effects of differentiation in 3-dimensional coculture on the level of genomic instability.
Document ID
Acquisition Source
Marshall Space Flight Center
Document Type
Conference Paper
Richmond, R.
(NASA Marshall Space Flight Center Huntsville, AL United States)
Kale, R.
(Alabama A & M Univ. Normal, AL United States)
Pettengill, O.
(Dartmouth Medical School Hanover, NH United States)
Rose, M. Franklin
Date Acquired
August 20, 2013
Publication Date
January 1, 2001
Subject Category
Life Sciences (General)
Meeting Information
Meeting: 2001 NASA Cell Science Conference
Location: Houston, TX
Country: United States
Start Date: March 7, 2001
Distribution Limits
Work of the US Gov. Public Use Permitted.

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