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Effect of 670-nm Light-Emitting Diode Light On Neuronal CulturesLight close to and within the near infrared range has documented benefits for promoting wound healing in human and animal studies. Our preliminary results using light-emitting diodes (LEDs) in this range have also demonstrated two-to five-fold increases in growth-phase-specific DNA synthesis in normal fibroblasts, muscle cells, osteoblasts, and mucosal epithelial cells in tissue cultures. However, the mechanisms of action of such light on cells are poorly understood. We hypothesized that the therapeutic effects of such light result from the stimulation of cellular events associated with increases in cytochrome oxidase activity. As a first step in testing our hypothesis, we subjected primary neuronal cultures to impulse blockade by tetrodotoxin (TTX), a voltage-dependent sodium channel blocker, and applied LED light at 670 nm to determine if it could partially or fully reverse the reduction of cytochrome oxidase activity by TTX. The wavelength and parameters were previously tested to be beneficial for wound healing.
Document ID
Document Type
Conference Paper
Wong-Riley, Margaret T. T.
(Medical Coll. of Wisconsin Milwaukee, WI United States)
Whelan, Harry T.
(Medical Coll. of Wisconsin Milwaukee, WI United States)
Date Acquired
August 21, 2013
Publication Date
October 1, 2002
Publication Information
Publication: Second International Conference on Near-Field Optical Analysis: Photodynamic Therapy and Photobiology Effects
Subject Category
Life Sciences (General)
Funding Number(s)
Distribution Limits
Work of the US Gov. Public Use Permitted.
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