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Cellular Oxygen and Nutrient Sensing in Microgravity Using Time-Resolved Fluorescence MicroscopyOxygen and nutrient sensing is fundamental to the understanding of cell growth and metabolism. This requires identification of optical probes and suitable detection technology without complex calibration procedures. Under this project Microcosm developed an experimental technique that allows for simultaneous imaging of intra- and inter-cellular events. The technique consists of frequency-domain Fluorescence Lifetime Imaging Microscopy (FLIM), a set of identified oxygen and pH probes, and methods for fabrication of microsensors. Specifications for electronic and optical components of FLIM instrumentation are provided. Hardware and software were developed for data acquisition and analysis. Principles, procedures, and representative images are demonstrated. Suitable lifetime sensitive oxygen, pH, and glucose probes for intra- and extra-cellular measurements of analyte concentrations have been identified and tested. Lifetime sensing and imaging have been performed using PBS buffer, culture media, and yeast cells as a model systems. Spectral specifications, calibration curves, and probes availability are also provided in the report.
Document ID
Acquisition Source
Marshall Space Flight Center
Document Type
Contractor or Grantee Report
Szmacinski, Henryk
(Microcosm, Inc. Columbia, MD, United States)
Date Acquired
August 21, 2013
Publication Date
August 15, 2003
Subject Category
Instrumentation And Photography
Funding Number(s)
Distribution Limits
Work of the US Gov. Public Use Permitted.
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