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A method for the 32P labeling of peptides or peptide nucleic acid oligomersA novel approach to the radioactive labeling of peptides and PNA oligomers is described. It is based on the conjugation of a deoxynucleoside 3'-phosphate with the terminal amine of the substrate, followed by phosphorylation of the 5'-hydroxyl group of the nucleotide using T4 polynucleotide kinase and [gamma-32P]ATP.
Document ID
20040172628
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
External Source(s)
doi:10.1021/bc980035c
Authors
Kozlov, I. A. (The Salk Institute for Biological Studies P.O. Box 85800, San Diego, California 92186, United States)
Nielsen, P. E.
Orgel, L. E.
Bada, J. L.
Date Acquired
August 22, 2013
Publication Date
July 1, 1998
Publication Information
Publication: Bioconjugate chemistry
Volume: 9
Issue: 4
ISSN: 1043-1802
Subject Category
Exobiology
Report/Patent Number
ISSN: 1043-1802
Distribution Limits
Public
Copyright
Other
Keywords
Non-NASA Center
NASA Discipline Exobiology
Oligonucleotides/chemistry
Phosphorus Radioisotopes/chemistry
Peptides/chemistry
Isotope Labeling/methods
Chromatography, High Pressure Liquid
Support, U.S. Gov't, Non-P.H.S
DNA Ligases/chemistry
Phosphorylation
Adenosine Triphosphate/chemistry

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