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Analysis of Cell Proliferation in Newt (Pleurodeles waltl) Tissue Regeneration during Spaceflight in Foton M-2Terrestrial organisms exposed to microgravity during spaceflight expe rience musculoskeletal degeneration. It is still not understood if lo nger-term exposures to microgravity induce degeneration in other tiss ues, and if these effects are also observed in neutrally buoyant aqu atic organisms that may be pre-adapted to mechanical unloading. The " Regeneration" experiment conducted collaboratively between Russian an d US scientists for 16 days in the Russian Foton M-2 spaceflight soug ht to test the hypothesis that microgravity alters the proliferation of cells in regenerating tail tissue of the newt Pleurodeles waltl. Our initial results indicate that we successfUlly delivered the proli feration marker 5-bromo-2'-deoxy Uridine (BrdU) during spaceflight, and that it was incorporated in the nuclei of cells in regenerating tis sues. Cells in spaceflight tail regenerates proliferated at a slight ly slower rate and were more undifferentiated than those in ground sy nchronous controls. In addition, the size of regenerating tails from spaceflight was smaller than synchronous controls. However, onboard temperature recordings show that the temperature in spaceflight was a bout 2 C lower than ground synchronous controls, possibly explaining the observed differences. Additional post-facto ground controls at ma tched temperatures will correctly determine the effects of spaceflig ht on regenerative cell proliferation in the newt.
Document ID
20070022665
Acquisition Source
Ames Research Center
Document Type
Reprint (Version printed in journal)
Authors
Almeida, E. A. C.
(NASA Ames Research Center Moffett Field, CA, United States)
Roden, C.
(NASA Ames Research Center Moffett Field, CA, United States)
Phillips, J. A.
(NASA Ames Research Center Moffett Field, CA, United States)
Yusuf, R.
(NASA Ames Research Center Moffett Field, CA, United States)
Globus, R. K.
(NASA Ames Research Center Moffett Field, CA, United States)
Searby, N.
(NASA Ames Research Center Moffett Field, CA, United States)
Vercoutere, W.
(NASA Ames Research Center Moffett Field, CA, United States)
Morey-Holton, E.
(NASA Ames Research Center Moffett Field, CA, United States)
Tairbekov, M.
(Academy of Sciences (Russia) Moscow, Russian Federation)
Grigoryan, N.
(Academy of Sciences (Russia) Moscow, Russian Federation)
Domaratskaya, E.
(Academy of Sciences (Russia) Moscow, Russian Federation)
Poplinskaya, V.
(Academy of Sciences (Russia) Moscow, Russian Federation)
Mitashov, V.
(Academy of Sciences (Russia) Moscow, Russian Federation)
Date Acquired
August 23, 2013
Publication Date
July 1, 2006
Publication Information
Publication: Journal of Gravitational Physiology, Volume 13, No. 1
Subject Category
Aerospace Medicine
Funding Number(s)
CONTRACT_GRANT: RFBR-04-04-48044
CONTRACT_GRANT: RFBR-05-04-48502
Distribution Limits
Public
Copyright
Other

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