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Apparatus for Cold, Pressurized Biogeochemical ExperimentsA laboratory apparatus has been devised as a means of studying plausible biogeochemical reactions under high-pressure, low-temperature aqueous, anaerobic conditions like those conjectured to prevail in a liquid water ocean on Europa (the fourth largest moon of the planet Jupiter). The experiments to be performed by use of this apparatus are intended to enhance understanding of how life (if any) could originate and evolve in the Europa ocean environment. Inasmuch as terrestrial barophilic, psychrophilic organisms that thrive under anaerobic conditions are used in the experiments, the experiments may also contribute to terrestrial biogeochemistry. The apparatus (see figure) includes a bolt-closure reaction vessel secured inside a refrigerator that maintains a temperature of 4 C. Pressurized water is supplied to the interior of the vessel by a hydrostatic pump, which is attached to the vessel via high-pressure fittings. The terrestrial organisms used in the experiments thus far have been several facultative barophilic, psychrophilic stains of Shewanella bacteria. In the experiments, these organisms have been tested for reduction of ferric ion by growing them in the presence of a ferric food source under optimized terrestrial conditions. The short-term goal of these experiments has been to select Shewanella strains that exhibit iron-reduction capability and test their ability to facilitate biogeochemical reduction of iron under temperature and pressure conditions imitating those in Europa s ocean. It is anticipated, that, once growth under Europa-like conditions has been achieved, the selected Shewanella strains will be used to facilitate biogeochemical reactions of sulfate and carbonate with hydrogen gas. Any disequilibrium of the products with the environment would be interpreted as signifying biogenic activity and the possibility of life in Europa s ocean.
Document ID
Document Type
Other - NASA Tech Brief
Amashukeli, Xenia (California Inst. of Tech. Pasadena, CA, United States)
Pappalardo, Robert T. (California Inst. of Tech. Pasadena, CA, United States)
Connon, Stephanie A. (California Inst. of Tech. Pasadena, CA, United States)
Gleeson, Damhnait F. (Colorado Univ. CO, United States)
Date Acquired
August 25, 2013
Publication Date
March 1, 2010
Publication Information
Publication: NASA Tech Briefs, March 2010
Subject Category
Man/System Technology and Life Support
Report/Patent Number
Distribution Limits
Public Use Permitted.

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IDRelationTitle20100009649Analytic PrimaryNASA Tech Briefs, March 2010