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Engineering of Methane Metabolism in Pichia Pastoris Through Methane Monooxygenase ExpressionExploration of the solar system is constrained by the cost of moving mass off Earth. Producing materials in situ will reduce the mass that must be delivered from earth. CO2 is abundant on Mars and manned spacecraft. On the ISS, NASA reacts excess CO2 with H2 to generate CH4 and H2O using the Sabatier System. The resulting water is recovered into the ISS, but the methane is vented to space. Thus, there is a capability need for systems that convert methane into valuable materials. Methanotrophic bacteria consume methane but these are poor synthetic biology platforms. Thus, there is a knowledge gap in utilizing methane in a robust and flexible synthetic biology platform. The yeast Pichia pastoris is a refined microbial factory that is used widely by industry because it efficiently secretes products. Pichia could produce a variety of useful products in space. Pichia does not consume methane but robustly consumes methanol, which is one enzymatic step removed from methane. Our goal is to engineer Pichia to consume methane thereby creating a powerful methane-consuming microbial factory.
Document ID
20180000684
Acquisition Source
Ames Research Center
Document Type
Presentation
Authors
Fleury, Samantha T.
(Universities Space Research Association Moffett Field, CA, United States)
Neff, Lily S.
(Wesley College Dover, DE, United States)
Galazka, Jonathan M.
(NASA Ames Research Center Moffett Field, CA, United States)
Date Acquired
January 24, 2018
Publication Date
September 28, 2017
Subject Category
Inorganic, Organic And Physical Chemistry
Life Sciences (General)
Report/Patent Number
ARC-E-DAA-TN47308
Meeting Information
Meeting: Ames Research and Technology Showcase (ARTS) Event
Location: Moffett Field, CA
Country: United States
Start Date: September 28, 2017
Sponsors: NASA Ames Research Center
Distribution Limits
Public
Copyright
Use by or on behalf of the US Gov. Permitted.
Keywords
Pichia
Synthetic Biolog
Methane
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