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Temporal RNA Integrity Analysis of Archived Spaceflight Biological Samples from ALSDA from 1991 to 2016The purpose of this study is to assess the quality of spaceflight tissues stored in Ames Life Science Data Archive (ALSDA) freezers. Garnering information for downstream functional analysis such as generation of omics datasets from tissues is, in part, dependent on the state of sample preservation. To assess the viability of a select group of tissues, RNA integrity number (RIN) values were calculated for RNA extracted from rodent livers. Rat livers from Spacelab Life Sciences 1 (SLS-1) and mouse livers from Commercial Biomedical Test Module 3 (CBTM-3), Rodent Research 1 (RR1), and Rodent Research 3 (RR3) were tested. It was found that mean RIN values from CBTM3, RR1, and RR3 were suitable for downstream functional analysis (RIN greater than 5) while the mean RIN value for SLS-1 was not (RIN equal to 2.5 plus or minus 0.1). Information from this study could lay the foundation for future efforts in determining the types of assays that are most appropriate for different tissues in ALSDA freezers, which would maximize the scientific return on rare spaceflight samples.
Document ID
20180007404
Acquisition Source
Ames Research Center
Document Type
Presentation
Authors
Degadillo Talburt, Elizabeth
(Santa Clara Univ. CA, United States)
French, Alison J.
(Wyle Labs., Inc. Moffett Field, CA, United States)
Lopez, Danielle K.
(Wyle Labs., Inc. Moffett Field, CA, United States)
Polo, San-Huei Lai
(Wyle Labs., Inc. Moffett Field, CA, United States)
Boyko, Valery
(Wyle Labs., Inc. Moffett Field, CA, United States)
Dinh, Marie T.
(Wyle Labs., Inc. Moffett Field, CA, United States)
Rask, Jon C.
(Wyle Labs., Inc. Moffett Field, CA, United States)
Kaushik, Chakravarty
(Logyx, LLC Mountain View, CA, United States)
Stewart, Helen J.
(NASA Ames Research Center Moffett Field, CA, United States)
Date Acquired
November 1, 2018
Publication Date
May 20, 2018
Subject Category
Life Sciences (General)
Report/Patent Number
ARC-E-DAA-TN55906
Meeting Information
Meeting: International Society for Biological and Environmental Repositories (ISBER) 2018 Annual Meeting & Exhibits
Location: Dallas, TX
Country: United States
Start Date: May 20, 2018
End Date: May 24, 2018
Sponsors: International Society for Biological and Environmental Repositories (ISBER)
Funding Number(s)
CONTRACT_GRANT: NNA14AB82C
CONTRACT_GRANT: OSSI
Distribution Limits
Public
Copyright
Public Use Permitted.
Keywords
ALSDA
Rodent Research
Biospecimens
Biobanking
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