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Nucleic acid hybridization with RNA immobilized on filter paper.RNA has been immobilized in a manner suitable for use in molecular hybridization experiments with dissolved RNA or DNA by a nonaqueous solid-phase reaction with carbonyldiimidazole and RNA 'dry coated' on cellulose or, preferably, on previously activated phosphocellulose filters. Immobilization of RNA does not appear to alter its chemical character or cause it to acquire affinity for unspecific RNA or DNA. The versatility and efficiency of this method make it potentially attractive for use in routine analytical or preparative hybridization experiments, among other applications.
Document ID
19720062107
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
Authors
Saxinger, W. C.
Ponnamperuma, C.
(Maryland, University College Park, Md., United States)
Gillespie, D.
(National Institutes of Health, Laboratory of Molecular Aging, Bethesda; Baltimore City Hospitals, Baltimore Md., United States)
Date Acquired
August 6, 2013
Publication Date
October 1, 1972
Publication Information
Publication: National Academy of Sciences
Subject Category
Chemistry
Accession Number
72A45773
Funding Number(s)
CONTRACT_GRANT: NGR-21-002-317
Distribution Limits
Public
Copyright
Other

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