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Applications of cryogenics in electron microscopyDescription of research and development efforts which resulted in a high-voltage cryoelectron microscope system capable of consistent operation at 1.8 to 4.2 K. Attention is given to the design and operation of superconducting objective lenses providing enhanced resolution during longer exposure times at lower beam intensities (thus reducing radiation damage of specimens). A specific system described combines a closed-cycle superfluid helium refrigerator integrated with a modified 200 kV electron microscope. Consistent resolutions of 8 to 16 A are attained with significantly reduced radiation damage, contamination, and thermal noise in prolonged vibration-free examination of specimens at temperatures from 1.8 to 4.2 K. Applications in specific disciplines are discussed, including membrane ultrastructure, cryobiology, microelectronics, and general superconductivity research.
Document ID
19740041095
Acquisition Source
Legacy CDMS
Document Type
Other - Collected Works
Authors
Fernandez-Moran, H.
(Chicago, University Chicago, Ill., United States)
Date Acquired
August 7, 2013
Publication Date
January 1, 1973
Subject Category
Instrumentation And Photography
Accession Number
74A23845
Funding Number(s)
CONTRACT_GRANT: NGL-14-001-012
CONTRACT_GRANT: NGR-14-001-166
CONTRACT_GRANT: PHS-GM-13243
CONTRACT_GRANT: PHS-GM-18236
Distribution Limits
Public
Copyright
Other

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