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Irregular bilayer structure in vesicles prepared from Halobacterium cutirubrum lipidsFluorescent probes were used to study the structure of the cell envelope of Halobacterium cutirubrum, and, in particular, to explore the effect of the heterogeneity of the lipids in this organism on the structure of the bilayers. The fluorescence polarization of perylene was followed in vesicles of unfractionated lipids and polar lipids as a function of temperature in 3.4 M solutions of NaCl, NaNO3, and KSCN, and it was found that vesicles of unfractionated lipids were more perturbed by chaotropic agents than polar lipids. The dependence of the relaxation times of perylene on temperature was studied in cell envelopes and in vesicles prepared from polar lipids, unfractionated lipids, and mixtures of polar and neutral lipids.
Document ID
19740062562
Document Type
Reprint (Version printed in journal)
Authors
Lanyi, J. K. (NASA Ames Research Center Biological Adaptation Branch, Moffett Field, Calif., United States)
Date Acquired
August 7, 2013
Publication Date
January 1, 1974
Publication Information
Publication: Biochimica et Biophysica Acta
Volume: 356
Subject Category
BIOSCIENCES
Distribution Limits
Public
Copyright
Other