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Comparison of media for detection of fungi on spacecraftFive media, including Trypticase soy agar (TSA; BBL) pour plates, spread plates of TSA, Mycophil agar with chloromycetin, Mycophil agar with chloromycetin and Actidione, and cornmeal agar with chloromycetin were quantitatively and qualitatively compared for the detection of fungi on spacecraft. Cornmeal agar with chloromycetin yielded the highest number of fungal colonies, although not always significantly higher than Mycophil agar with chloromycetin or TSA spread plates. Cornmeal agar with chloromycetin also gave the best qualitative representation of fungi on the spacecraft, recovering 68% of the genera found from all media. This medium yielded 10 times the number of fungal colonies and 3 times the number of genera found on TSA pour plates as currently used for spacecraft assay.
Document ID
19740062563
Document Type
Reprint (Version printed in journal)
Authors
Herring, C. M.
Brandsberg, J. W.
Oxborrow, G. S.
Puleo, J. R. (U.S. Public Health Service, Center for Disease Control, Cape Canaveral Fla., United States)
Date Acquired
August 7, 2013
Publication Date
March 1, 1974
Publication Information
Publication: Applied Microbiology
Volume: 27
Subject Category
BIOTECHNOLOGY
Funding Number(s)
CONTRACT_GRANT: NASA ORDER W-13062
Distribution Limits
Public
Copyright
Other