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Record 39 of 384
Quantitative bioluminescent detection of bacteria
Author and Affiliation:
Chappelle, E. W.
Picciolo, G. L.
Abstract: Phosphoflavins in sample are measured using photobacterial luciferase assay technique for flavin mononucleotide (FMN). Boiling perchloric acid is used to rupture cells to free bound flavin and to hydrolyze flavin adenine dinucleotide to FMN. Base-stabilized water solution of sodium borohydride is used as reactant.
Publication Date: Mar 01, 1976
Document ID:
19760000073
(Acquired Nov 04, 1995)
Accession Number: 76B10073
Subject Category: LIFE SCIENCES
Report/Patent Number: GSC-12003
Document Type: NASA Tech Brief
Publication Information: NASA Tech Briefs (ISSN 0145-319X); 1; 1; P. 81
Publisher Information: United States
Financial Sponsor: NASA; United States
Organization Source: NASA Goddard Space Flight Center; Greenbelt, MD, United States
Description: In English
Distribution Limits: Unclassified; Publicly available; Unlimited
Rights: No Copyright
NASA Terms: ASSAYING; BACTERIA; BACTERIOLOGY; BIOLUMINESCENCE; CHEMICAL ANALYSIS; ECOLOGY; OPTICAL MEASURING INSTRUMENTS; PHOTOMULTIPLIER TUBES
Availability Source: National Technology Transfer Center (NTTC), Wheeling, WV
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