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Functional polymeric microspheres based on 2-hydroxyethyl methacrylate for immunochemical studiesCo gamma irradiation of 2-hydroxyethyl methacrylate in the presence or in the absence of other acrylic monomers was found to constitute an effective technique for the synthesis of hydrophilic functional microspheres in the size range of approximately 0.3 to 3 microns in diameter. The effect of monomer concentration, steric stabilization, and electrostatic interaction on the particle size was investigated. Experimental conditions were determined to obtain desired particle sizes of relatively narrow distribution. It was shown that particles may be formed without intermediate micelles, i.e., by homogeneous nucleation, and the rate of particle formation is affected primarily by the rate of particle coalescence in the initial stages of the reaction. When covalently bound to antibodies these microspheres were successfully used to label murine and human lymphocytes.
Document ID
19770033828
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
Authors
Rembaum, A.
(California Inst. of Tech. Pasadena, CA, United States)
Yen, S. P. S.
(California Inst. of Tech. Pasadena, CA, United States)
Cheong, E.
(California Inst. of Tech. Pasadena, CA, United States)
Wallace, S.
(California Institute of Technology, Jet Propulsion Laboratory, Pasadena Calif., United States)
Molday, R. S.
(California Inst. of Tech. Pasadena, CA, United States)
Gordon, I. L.
(California Inst. of Tech. Pasadena, CA, United States)
Dreyer, W. J.
(California Institute of Technology Pasadena, Calif., United States)
Date Acquired
August 9, 2013
Publication Date
April 1, 1976
Publication Information
Publication: Macromolecules
Volume: 9
Subject Category
Chemistry And Materials (General)
Accession Number
77A16680
Funding Number(s)
CONTRACT_GRANT: NAS7-100
CONTRACT_GRANT: NIH-NC1-CB-43875
CONTRACT_GRANT: PHS-CA-15926
CONTRACT_GRANT: PHS-GM-06965
Distribution Limits
Public
Copyright
Other

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