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A technique for extracting blood samples from mice in fire toxicity testsThe extraction of adequate blood samples from moribund and dead mice has been a problem because of the small quantity of blood in each animal and the short time available between the animals' death and coagulation of the blood. These difficulties are particularly critical in fire toxicity tests because removal of the test animals while observing proper safety precautions for personnel is time-consuming. Techniques for extracting blood samples from mice were evaluated, and a technique was developed to obtain up to 0.8 ml of blood from a single mouse after death. The technique involves rapid exposure and cutting of the posterior vena cava and accumulation of blood in the peritoneal space. Blood samples of 0.5 ml or more from individual mice have been consistently obtained as much as 16 minutes after apparent death. Results of carboxyhemoglobin analyses of blood appeared reproducible and consistent with carbon monoxide concentrations in the exposure chamber.
Document ID
19770036519
Document Type
Reprint (Version printed in journal)
Authors
Bucci, T. J. (San Francisco Univ. CA, United States)
Hilado, C. J. (San Francisco Univ. CA, United States)
Lopez, M. T. (San Francisco, University San Francisco, Calif., United States)
Date Acquired
August 9, 2013
Publication Date
November 1, 1976
Publication Information
Publication: Journal of Combustion Toxicology
Volume: 3
Subject Category
LIFE SCIENCES (GENERAL)
Funding Number(s)
CONTRACT_GRANT: NSG-2039
Distribution Limits
Public
Copyright
Other