Microbiological profiles of the Viking spacecraft

Planetary quarantine requirements associated with the launch of two Viking spacecraft necessitated microbiological assessment during assembly and testing at Cape Canaveral and the Kennedy Space Center. Samples were collected from the Viking Lander Capsules (VLC), Orbiters (VO), and Shrouds at predetermined intervals during assembly and testing. Levels of bacterial spores per square meter on the VLC-1 and VLC-2 were 1.6 x 10 squared and 9.7, respectively, prior to dry-heat sterilization. The ranges of aerobic mesophilic microorganisms detected on the VO-1 and VO-2 were 4.2 x 10 squared to 4.3 x 10 cubed and 2.3 x 10 squared to 8.9 x 10 cubed/sq m, respectively. Approximately 1300 colonies were picked from culture plates, identified, lyophilized, and stored for future reference. About 75% of all isolates were microorganisms considered indigenous to humans; the remaining isolates were associated with soil and dust. The percentage of microorganisms of human origin was consistent with results obtained with previous automated spacecraft but slightly lower than those observed for manned (Apollo) spacecraft.