Computer simulation and experimental validation of isoelectric focusing in ampholine-free systems

An abbreviated version of a mathematical model of the steady state in isoelectric focusing is presented. Details of the mathematical transformations leading to a model suitable for computer implementation and numerical solution are given in the Appendix. The model describes the structure of concentration, pH, conductivity and potential gradients arising from the focusing of electrochemically defined ampholytes. Some of the results of computer simulations of two and three component systems are of particular interest to the experimentalist and are presented together with experimental validation of this model.